Table 1.
Plasmids and viruses used in this study.
| Name | Parental | Characteristics |
|---|---|---|
| Viruses (TMEV derivatives) | ||
| KJ6 | TMEV DA1 | LWT; L929 cell-adapted capsid |
| FB09 | TMEV KJ6 | LM60V; L929 cell-adapted capsid |
| Lentiviral and expression vectors 1 | ||
| pTM945 | pCCLsin | PromCMV-Empty-IRES-mCherry |
| pTM952 | pCCLsin | PromCMV-Empty-IRES-neo |
| pYH05 | pTM945 | PromCMV-huPKR-IRES-mCherry |
| pYH12 | pTM952 | PromCMV-huPKR-IRES-neo |
| pYH31 | pTM945 | PromCMV-huPKR(S6A)-IRES-mCherry |
| pYH32 | pTM945 | PromCMV-huPKR(S6D)-IRES-mCherry |
| pYH35 | pTM952 | PromCMV-huPKR(S6D)-IRES-neo |
| pTC20 | pTM945 | PromCMV-huPKR(S6AS97A)-IRES-mCherry |
| pTC21 | pTM945 | PromCMV-huPKR(S6DS97D)-IRES-mCherry |
| pTC22 | pTM945 | PromCMV-huPKR(S6AS97A4KA)-IRES-mCherry |
| pTC23 | pTM945 | PromCMV-huPKR(4KA)-IRES-mCherry |
| pTC24 | pTM952 | PromCMV-huPKR(S97D)-IRES-neo |
| pTC25 | pTM952 | PromCMV-huPKR(S6DS97D)-IRES-neo |
| pTC27 | pTM952 | PromCMV-huPKR(K296R)-IRES-neo |
| pSO1 | pTM945 | PromCMV-huPKR(S97A)-IRES-mCherry |
| pSO2 | pTM945 | PromCMV-huPKR(S97D)-IRES-mCherry |
| pFW29 | pTM952 | PromCMV-huPKR_SmBit-IRES-neo |
| pFW33 | pTM952 | PromCMV-huPKR_LgBit-IRES-neo |
| pFW39 | pTM952 | PromCMV-huPKR(S6AS97A)SmBit-IRES-neo |
| pFW40 | pTM952 | PromCMV-huPKR(S6DS97D)SmBit-IRES-neo |
| pFW41 | pTM952 | PromCMV-huPKR(4KA)SmBit-IRES-neo |
| pFW42 | pTM952 | PromCMV-huPKR(S6AS97A)LgBit-IRES-neo |
| pFW43 | pTM952 | PromCMV-huPKR(S6DS97D)LgBit-IRES-neo |
| pFW44 | pTM952 | PromCMV-huPKR(4KA)LgBit-IRES-neo |
| CRISPR-Cas9 vectors | ||
| pX461 | pSpCas9n(BB)-T2A-GFP | |
| pYH1 | pX461 | sgRNA1(exon1huPKR) |
| pYH2 | pX461 | sgRNA2(exon1huPKR) |
| pFW19 | pX461 | sgRNA1(exon4huPACT) |
| pFW20 | pX461 | sgRNA2(exon4huPACT) |
These plasmids were generated in a lentiviral vector backbone. They are named pTM, pYH, pTC… when used as expression plasmids, and TM, YH, TC… when used as lentiviral vectors.