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. 2021 Jan 20;53(1):136–149. doi: 10.1038/s12276-020-00552-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–f Dot-plot graph depicting cytokine/chemokine genes that are differentially expressed between the NTM patients and the HCs. Human PBMCs were isolated from the HCs (n = 39) and the NTM patients (Mabc, n = 31; Mmass, n = 22), and cytokine/chemokine mRNA levels were determined using quantitative real-time PCR analysis (a IL1B; b TNF; c IL6; d CXCL2; e CCL2; f CCL5). g–i Correlation matrix between cytokine/chemokine variables from the HCs (g), Mabc-infected patients (h), and Mmass-infected patients (i). A correlation matrix showed correlation coefficients for a combination of 6 cytokine/chemokine variables for all subjects in this study. ns, not significant. Mann–Whitney U test. Data are the combined results (mean ± SEM) from three or four independent experiments performed in duplicate. HC healthy controls, Mabc M. abscessus subsp. abscessus, Mmass M. abscessus subsp. massiliense.