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. 2021 Apr 28;11:9189. doi: 10.1038/s41598-021-88497-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Number of hepatocytes per µm² (b) hepatocytes area in µm², (c) liver mass corrected by tibia length (g/cm), (d) collagen content (%) analyzed using CellProfiler 3.1.5 in control (C), control + probiotic (CP), diabetic (D) and diabetic + probiotic (DP) groups (n = 6–9/group). Data are from C57BL/6 STZ-induced diabetic mice, + /− Saccharomyces boulardii (0.5 × 10⁸ colony-forming units, THT 500,101 strain, Probiotics and Starters Cultures, Belgium) for 8 weeks. A horizontal line inside the box indicates the median value of the samples within each group, and the upper and lower edges of the box indicate the quartiles. For (b,d) significance was determined by Kruskal–Wallis followed by Dunn’s test, and for (a,c) significance was determined by Two-way ANOVA followed by Tukey test for multiple comparisons of means (GraphPad Prism 6). + indicates mean value. **** p ≤ 0.0001.