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. 2021 Apr 28;11:9220. doi: 10.1038/s41598-021-88677-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry analysis of SHIP-1 expression in tissue cells. Cells were prepared from spleen and lung tissues of WT and Tek-Cre/SHIP-1 mice 3–8 weeks of age and stained with labeled antibodies to cell markers (CD45 and CD19) and intracellular SHIP-1 and analyzed by flow cytometry. (A) Splenocytes from WT and Tek-Cre/SHIP-1 mice stained with anti-CD45 and anti-SHIP-1 antibodies; (B) CD45 + gated splenocytes stained with anti-CD19 and anti-SHIP-1; (C) Lung tissue cells from WT and Tek-Cre/SHIP-1 mice stained with anti-CD45 and anti-SHIP-1. Shown are representative results of 3 separate experiments. Numbers are percentages of cells.