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. 2021 Apr 28;11:9220. doi: 10.1038/s41598-021-88677-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pulmonary histopathology of Tek-Cre/SHIP-1 mice. Shown are Hematoxylin and eosin (H&E) stained lung sections from (A) WT, VEC-Cre/SHIP-1, and Tek-Cre/SHIP-1 mice and (B) Tek-Cre/SHIP-1 mice. Lung sections from WT and VEC-Cre/SHIP-1 mice showed normal structure of airways and alveoli. In contrast, mild, medium, and severe cellular infiltration was seen in the airways and lung parenchyma of Tek-Cre/SHIP-1 mice. All Tek-Cre/SHIP-1 mice examined displayed inflammatory lung pathology. Pathology scores were assigned by a pathologist and through application of the NIH ImageJ software and its associated color deconvolution plugin.