In vitro |
|
|
|
|
Conditioned media |
CM from HEK293 cells expressing TDP-43 |
HEK293 cells, primary mouse cortical neuron |
O |
84 |
CM from HEK293 cells treated with ALS insoluble factions |
HEK293 cells |
X |
86 |
CM from HEK293 cells expressing wild-type TDP-43 or mutant TDP-43 ΔNLS |
HEK293 cells, primary mouse spinal cord cultured cells |
X |
87 |
Co-culture system |
SH-SY5Y cells harboring phosphorylated TDP-43 aggregates |
SH-SY5Y cells |
O |
85 |
HEK293 cells containing pTDP-43 aggregates |
HEK293 cells |
O |
86 |
HEK293 cells expressing TDP-43 |
HEK293 cells |
O |
84 |
SH-SY5Y cells expressing HA-tagged TDP-43 |
SH-SY5Y cells |
O |
88 |
NSC-34 cells expressing wild-type TDP-43-tdTomato |
NSC-34 cells expressing mutant TDP-43 G294A-tGFP |
O |
89 |
Using brain lysate or CSF from patients |
Insoluble fraction from brain tissue of ALS or FTLD-TDP patient |
SH-SY5Y cells |
O |
85 |
ALS patient-derived cerebellum or cortex lysate |
Mouse primary cortical neuron |
Only in case of treatment of cortex lysate |
84 |
Insoluble fraction from CNS tissues of ALS patient |
HEK293 cells, NSC-34 cells |
O |
86 |
Insoluble fraction from brain tissue of FTLD-TDP patient |
HEK293 cells expressed TDP-43 |
O |
90 |
CSF-derived ALS or FTD patient |
U251 cells |
O |
91 |