Fig. 6. The CRL4COP1–ETV5 axis operates in human islets and β cells.
a–c Effects of CRL inhibition (a), and COP1 (b), or ETV5 (c) knockdown on insulin secretion from isolated human islets (n = 3). Data are presented as mean ± SEM. P values were calculated by two-tailed Student’s t test. d High glucose medium depletes ETV5 levels in isolated human islets. An asterisk (*) indicates a nonspecific band. e–g Effects of CRL inhibition (e), and COP1 (f) or ETV5 (g) knockdown on insulin secretion from the EndoC-βH1 human beta cells (n = 6). Data are presented as mean ± SEM. P values were calculated by two-tailed Student’s t test. h High glucose medium depletes ETV5 levels in EndoC-βH1 cells.