Skip to main content
. 2020 Aug 11;52(8):1310–1325. doi: 10.1038/s12276-020-0475-0

Fig. 7. DANCR overexpression suppressed the promoting effect of the miR-320a inhibitor on the β-catenin signaling pathway.

Fig. 7

BMSCs were infected with lentivirus encoding miR-320a inhibitor and/or DANCR overexpression vector and further incubated in osteogenic differentiation inducing medium for 7 or 14 days. a Cells were lysed, and western blot analysis was performed to analyze the protein expression of β-catenin in the cytoplasm and nucleus, TCF-1, RUNX2, OPN, and OCN. b Quantitative analysis of protein expression in a. c Relative mRNA levels of TCF-1, RUNX2, OPN, and OCN were evaluated by qRT-PCR. All experiments were conducted independently at least three times. *P < 0.05 and **P < 0.01.