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. 2021 Apr 28;12:2456. doi: 10.1038/s41467-021-22812-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Mutations of RAF genes in the high-order mutants used in this study. b Photographs of seedlings after 4 weeks of growth in the soil. Bar = 1 cm. c Photographs of seedlings after 10 days of germination and growth on 1/2 MS medium containing indicated concentrations of ABA, without (upper panel) or with (bottom panel) 1% sucrose. The position of mutants in the image is shown in the box at the upper-right corner. d Photographs of seedlings after 10 days of germination and growth on 1/2 MS medium containing indicated concentrations of ABA without sucrose. e Photographs of seedlings after 10 days of germination and growth on 1/2 MS medium containing indicated concentrations of ABA with 1% sucrose. f The ABA-induced phosphorylation of Ser171 and Ser175 of SnRK2.6 in wild-type and the OK¹⁰⁰-B2 and OK¹⁰⁰-B3 mutants. The anti-pS171 and anti-pS175 immunoblots were used to show the phosphorylation of the conserved serine residues. Arrows indicate the non-specific bands recognized by anti-pS171 and anti-pS175 antibodies. Images shown are representative of at least two independent experiments. Source data are provided in Source Data.