Fig. 5. Transduction of lentivirus overexpressing miR-24-3p and/or miR-25-3p dampens the PER2::LUC rhythm in the neonatal SCN slice cultures obtained from the PER2::LUC knock in transgenic mice.

a Experimental design for transducing CMV-promoter driven lentivirus-miR-control/miR-24-3p/miR-25-3p-GFP in neonatal suprachiasmatic nucleus (SCN) slice cultures. b Representative results of PER2::LUC oscillation measured by a real-time bioluminescence recording device. The bioluminescence patterns were aligned to the first nadir of data acquired to compare changes in the expression patterns of PER2::LUC in the pre- and postlentiviral transduced SCN slice cultures (experiments were performed three independent times, n = 3). c Changes in PER2 amplitudes and periods are presented as the means ± SE. Significance was assessed by Student’s t test, *p < 0.01 compared to the prelentiviral transduction conditions.