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. 2020 Jul 8;52(7):1090–1101. doi: 10.1038/s12276-020-0460-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Regeneration kinetics of 11cRAL a, atRPalm b, and atRAL c were examined after ~90% rhodopsin bleaching by illumination at 24 mW/cm² for 2 min. Abca4^−/− mice kept in the dark for 24 h (DA) were administered BSA or BSA + IP-DHA (25 mg/kg) in the tail vein 5 min before the 2-min light exposure. Prior to (DA) or after bleaching (PB), the mice were returned to the dark for the indicated time (DA 1H, 24H, and 48H), and retinoid quantification was subsequently performed by normal-phase HPLC. Error bars represent the SD, n = 3.