Figure 3. Miniaturized SHP2 equipment workflow.
Routine performance of the assay makes use of the equipment indicated. Assay plates are prepared using an acoustic liquid handler that delivers nanoliter volumes of compounds. Cell transfer can be performed manually, using a multichannel pipette, or by using an automatic liquid dispenser. Heat pulse denaturation uses a thermocycler that can deliver thermal gradients to a 384-well plate. A microplate reader is used to detect the β-gal EFC assay chemiluminescence.