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. 2021 Mar 10;53(3):407–421. doi: 10.1038/s12276-021-00577-z

Fig. 4. The NF-κB pathway and Wnt signaling are upregulated in the livers of mice with LPS-induced endotoxemia.

Fig. 4

Protein and RNA were extracted from the liver tissues of mice injected with 0, 5, or 25 mg/kg LPS. a Western blotting was performed to measure the levels of phospho-IκB and IκB in total tissue lysates and the NF-κB protein in nuclear lysates. β-Actin and TATA box-binding protein (TBP) was used as loading controls for total tissue lysates and nuclear lysates, respectively. Western blot raw image data are provided in Supplementary Data 5. bd Band intensities were measured using ImageJ. e The binding activity of NF-κB to its target DNA sequence was measured using ELISA. fi Messenger RNA levels of major proinflammatory cytokines was measured by RT-qPCR. jm Messenger RNA levels of Wnt ligands were quantified by RT-qPCR. n Levels of the Wnt pathway components were measured by western blotting. β-Actin was used as the loading control. Western blot raw image data are provided in Supplementary Data 5. or Band intensities were quantified using ImageJ. The data are presented as the mean ± standard deviation values of quadruplicate experiments. Differences among experimental groups were analyzed using one-way ANOVA with Duncan’s multiple range test. The different letters indicate significant differences (*P < 0.05).