Fig. 7. LGK974 reduces the coimmunoprecipitation and colocalization of β-catenin and NF-κB in the liver tissues of endotoxemic mice.

a, b Protein was extracted from the liver tissues of mice injected with 0, 40, or 60 mg/kg LGK974 and 0 or 25 mg/kg LPS. The protein–protein interaction between β-catenin and NF-κB p65 was analyzed by coimmunoprecipitation (a). Input samples from the coimmunoprecipitation assay were analyzed by western blotting (b). IP immunoprecipitation, WB western blot. c Images showing immunofluorescence staining for β-catenin and NF-κB and DAPI staining for nuclei. All images were acquired at a magnification of 600× using a Nikon Asia confocal system (Nikon, Tokyo, Japan). Images are representative of three experiments. Colocalization was quantified using ImageJ to calculate Pearson correlation coefficient (R) values.