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. 2021 Mar 2;53(3):369–383. doi: 10.1038/s12276-021-00570-6

Fig. 6. ULK1 phosphorylation is upregulated in human pancreatic cancer cell lines.

Fig. 6

a, b Western blotting analysis of a normal human pancreatic cell line (HPNE, human pancreatic nestin-expressing cells) and cancer cell lines (BxPC3, MIA PaCa-2, and CFPAC) for endogenous levels of ULK1 phosphorylation at a S405 and b S415 with either S405 or S415 phospho-specific antibodies. The graph shows the quantification of p-ULK1 levels after normalization to total ULK1 (n = 4). c Inhibition of ULK1 phosphorylation at S405 and S415 by BIO for 2 h in BxPC3 and MIA PaCa-2 cells. d Effect of BIO on the survival of BxPC3 and MIA PaCa-2 cells upon EBSS starvation. (n = 5). In all experiments, the blots shown are representative of at least three experiments with similar results. *P < 0.05, **P < 0.01, and ***P < 0.001.