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. 2021 Feb 9;53(2):250–263. doi: 10.1038/s12276-021-00564-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Immunoprecipitation-based in vivo cellular methylation assay using anti-methylated lysine (anti-α-MeK) antibody revealed that SRF was methylated during C2C12 differentiation. Notably, methylation was increased in the cells cultured in differentiation medium (DM). b HA-tagged truncated mutants of SRF (SRF-A, SRF-AB, and SRF-C) were transfected into C2C12 cells, and then, the cells were subjected to serum deprivation (DM). The SRF MADS-box region was methylated, as determined with a α-MeK immunoprecipitation-based assay. c Transfection of SET7 induced the methylation of SRF in the C2C12 cells. d In vitro methyltransferase assay in complete cell-free conditions showing that the chimeric protein GST-SET7 induced the methylation of GST-SRF. GST protein served as a control. Left: Coomassie blue staining was used to determine the expression of GST proteins. Right: Autoradiograph. e Enzymatically dead SET7 (SET7 H297A) failed to induce the methylation of SRF. f Set7 siRNA attenuated the binding of SRF to the proximal SRE in the Acta1 gene promoter. ChIP analysis. g Expression of SET7 was increased during C2C12 differentiation. h Association between endogenous SET7 and SRF was enhanced in DM.