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. 2020 Dec 11;52(12):2005–2019. doi: 10.1038/s12276-020-00539-x

Fig. 3. Impact of direct perturbation of Ac-CoA pools on histone acylation.

Fig. 3

a Schematic presentation depicting the flux of metabolites mediated by ACLY, CRAT, and ACSS2, which contributes to Ac-CoA/SCA-CoA pools that support nuclear histone modification. b Relative cell count (%) of the indicated siRNA-transfected C2C12 myoblasts compared to the percentage of siCtl. The cells were analyzed 3 days after siRNA treatment. The error bars indicate the SEM (n = 5). Statistical significance was analyzed based on comparison to siCtl, *p < 0.05; **p < 0.01. c WB of total lysates obtained from C2C12 myoblasts transfected with the indicated siRNAs. d–e Heat maps of metabolite fold changes. C2C12 myoblasts were transfected with the indicated siRNAs for 72 h, and metabolite abundance was measured using GC–MS; each column indicates an individual sample (n = 3). Glycolysis, PPP, and TCA cycle intermediates (d) and fatty acids (e) are shown.