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. 2021 Apr 28;11:9147. doi: 10.1038/s41598-021-87402-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Examples of alternans maps and traces. (A) Calcium transient amplitude alternans map corresponding to discordant alternans, with warmer colors corresponding to alternans with large amplitude. Dark blue zones are nodal lines separating zones of alternans in opposite phases. The pixels marked by red arrows with annotations giving the row and column indices are used in the subsequent panel. (B) Sample traces from the three marked pixels in the recording in (A), verifying that the alternans is discordant (blue and yellow traces have opposite phases) and that there is a lack of alternans in the nodal lines (red trace). (C,D) Similar format to (A,B), but showing spatially heterogeneous alternans in an infarcted heart, where alternans arises only in the infarct border zone (BZ) but not the non-infarcted zone (NZ).