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. 2021 Jan 4;53(1):52–66. doi: 10.1038/s12276-020-00530-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Double immunofluorescence staining of Gli1, Gli2, Gli3 (red), and 11 β-HSD2 (green) in normal human first-trimester villous tissues, nuclei were counterstained with DAPI (blue). Scale bars, 20 μm. b Immunoblot analysis confirmed the Gli2 knockdown efficiency in sh-Ctrl-JAR cells and sh-Gli2-JAR cells. c Immunoblot analysis confirmed the Gli3 knockdown efficiency in sh-Ctrl-JAR cells and sh-Gli3-JAR cells. d After indicated treatments, migration and invasion of JAR cells were measured in matrigel cell invasion and transwell cell migration assays. Scale bars, 200 μm. e Migrated cell from d was quantified by Image J software, each group was normalized to “sh-Ctrl + Ctrl” group. f Invaded cell from panel d was quantified by Image J software, each group was normalized to “sh-Ctrl + Ctrl” group. *p < 0.05, **p < 0.01, ***p < 0.001.