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. 2021 Jan 4;53(1):52–66. doi: 10.1038/s12276-020-00530-6

Fig. 5. Inhibition of Shh signaling induces autophagy and autolysosome.

Fig. 5

a After treating with or without indicated dose of chloroquine (CQ), cyclopamine and/or Gant61 in JAR cells for 24 h, protein levels of LC3B and GAPDH were measured by western blot assays. b Expression of LC3B/GAPDH from panel a was quantified by ImageJ software. c, d JAR cells were infected with GFP-mCherry-LC3 adenovirus vector (5 × 109 pfu/ml) more than 24 h, followed with Gant61 (10 μM) with or without CQ for 24 h, mCherry-positive GFP-negative (mCherry+ GFP−) puncta were captured by microscope and quantified by ImageJ software. Scale bar, 50 μm. **p < 0.01. e, f After treating with Gant61 (10 μM), colocalization of LC3 and LAMP1 in JAR cells captured by microscope and quantified by Image J software. Scale bars, 20 μm. *p < 0.05, **p < 0.01, ***p < 0.001.