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. 2020 Dec 14;52(12):2034–2045. doi: 10.1038/s12276-020-00545-z

Fig. 2. Spermidine inhibits senescence in BLM-induced mouse lung and alveolar epithelial cells.

Fig. 2

a. Mouse primary AECs were exposed to BLM (10 µg/mL) and/or spermidine (100 µM) for 24 h in serum-free medium. β-gal staining showed increased SA-β-gal activity in AECs after BLM exposure, and treatment with 100 µM spermidine reversed senescence in these cells. Scale bar, 50 μm. Mice were treated with BLM (3 U/kg) intratracheally on day 0, and spermidine (50 mg/kg/mouse/day) was administered intraperitoneally on days 10–21. Lung samples were collected on day 21. b Immunoblot analyses of the expression of p16 and p21 in whole lungs from mice. The intensity of each band was quantified by densitometry, and the data were normalized to β-actin. *p < 0.05 vs. the BLM(+)/spermidine(−)group. (n = 3) c Photographs of immunohistochemical staining showing the expression of p16 and p21 in mouse lungs. Original magnification ×100.