Table 1.
Reagent | μl/step | Steps | μl/cycle | μl/20 cycles | ml/platea of 1536 samples | Fold-lower than 96-AMOS |
---|---|---|---|---|---|---|
TCA | 3.5 | 3 | 10.5 | 210 | 322.56 | 27 |
Base | 1 | 1 | 1 | 20 | 30.72 | 20 |
Activator | 1.5 | 1 | 1.5 | 30 | 46.08 | 18 |
Cap | 2 | 1 | 2 | 40 | 61.44 | 13 |
Oxidizer | 3 | 1 | 3 | 60 | 92.16 | 7 |
Wash | 4 | 5 | 20 | 400 | 614.40 | 58 |
Argon | 4b | 113 | ||||
Yieldc | 3.5 | |||||
Cost/based | 0.00277 |
Reagent consumption on NG-1536-AMOS platform compared with 96-AMOS. For example, 3.5 μl of TCA are delivered per well (10.5 μl over 3 steps). A total of 210 μl of TCA are delivered over 20 cycles (to generate a 20-base strand); 322.56 ml total TCA are delivered to 1536 wells over 20 cycles. Reagent consumption of TCA on NG-1536-AMOS compared to 96-AMOS is 27-fold lower.
20-Base strands per well.
Pounds of argon per 20 cycles.
Average yield (nanomoles) over 50 samples.
Cost/base including labor; the washing reagent “Wash” used for synthesis is acetonitrile.
Each capping step is composed of Cap A and B at 1 μl each (total 2 μl).