Table 1.

NG-1536-AMOS reagent consumption, yield and cost per base.

Reagent	μl/step	Steps	μl/cycle	μl/20 cycles	ml/platea of 1536 samples	Fold-lower than 96-AMOS
TCA	3.5	3	10.5	210	322.56	27
Base	1	1	1	20	30.72	20
Activator	1.5	1	1.5	30	46.08	18
Cap	2	1	2	40	61.44	13
Oxidizer	3	1	3	60	92.16	7
Wash	4	5	20	400	614.40	58
Argon					4b	113
Yieldc	3.5
Cost/based	0.00277

Reagent consumption on NG-1536-AMOS platform compared with 96-AMOS. For example, 3.5 μl of TCA are delivered per well (10.5 μl over 3 steps). A total of 210 μl of TCA are delivered over 20 cycles (to generate a 20-base strand); 322.56 ml total TCA are delivered to 1536 wells over 20 cycles. Reagent consumption of TCA on NG-1536-AMOS compared to 96-AMOS is 27-fold lower.

^a20-Base strands per well.

^bPounds of argon per 20 cycles.

^cAverage yield (nanomoles) over 50 samples.

^dCost/base including labor; the washing reagent “Wash” used for synthesis is acetonitrile.

Each capping step is composed of Cap A and B at 1 μl each (total 2 μl).