Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 28;12(5):420. doi: 10.1038/s41419-021-03680-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A The expression profiles of the top 39 differentially expressed circRNAs in MDA-MB-231 cells (TNBC cell line) and their exosomes compared with MCF-7 cells (non-TNBC cell line) and their exosomes. Pseudo-colors (green to red) indicate the expression levels from low to high. B The significantly differentially expressed circRNAs represent in Circos diagrams generated by Circos (http://circos.ca/) with the threshold criteria of fold change > 2.0 and P < 0.05. Red bars indicate upregulated circRNAs, while blue bars represent downregulated circRNAs. The height of the bar means the fold change of the expression level of a circRNA. C Representative images of exosomes from MDA-MB-231 cells and MCF-7 cells using a transmission electron microscope (TEM). Scale bar, 100 nm. D The expression level of circPSMA1 was verified in serum exosome samples from TNBC patients (n = 20) and non-TNBC patients (n = 20) by qRT-PCR analysis. E The expression level of circPSMA1 was verified in TNBC cell lines (BT-549 and MDA-MB-231), non-TNBC cell lines (MCF/7 and MCF-10A). F The schematic illustrates the regions of qRT-PCR primers for the genomic loci of PSMA1 gene and circPSMA1. Convergent primer for detecting linear PSMA1 and divergent primers for circPSMA1. G Gel electrophoresis analysis of RT-PCR for the existence of circPSMA1 in breast cancer cells. CircPSMA1 is only amplified by divergent primers in cDNA treated with RNase. H The genomic loci of PSMA1 gene and circPSMA1. Red arrow indicates the back-splicing region of PSMA1 exon 3 to exon 7 verified by Sanger sequencing. The circPSMA1 spliced mature sequence length is 411 nt. I The FISH assay shows the subcellular localization of circPSMA1. The circPSMA1 probe is labeled with Cy3 (red) and nuclei is stained with Hochest 33342 (blue) (magnification, ×200, Scale bar = 20 μm). All data were shown as mean ± SD at least three independent experiments, *p < 0.05, ***p < 0.001.