FIG. 7.

Gene expression cluster analysis of early human retinal organoids (week 10) from hESCs line H1 and human fetal retina at weeks 8, 10, 11, 13, and 16. (a) Pearson's correlation coefficient for 10 week-old retinal organoid and 8-, 10-, 11-, 13-, and 16 week-old fetal retina shows similarity in gene expression between 10 week-old retinal organoid and 8–10 week-old fetal retina. The X and Y axis represent each sample. The color represents the correlation coefficient (the darker the color, the higher the correlation; the lighter the color, the lower the correlation). Heatmaps illustrate gene expression profiles of retinal progenitor cell (b), retinal ganglion cell (c), retinal pigment epithelial cell (d), rod PR cell (e), and cone PR cell (f) in early retinal organoid and human fetal retina. RNA-Seq data show that most retinal progenitor cell genes, retinal ganglion cell and cone PRs exhibit comparable expression pattern between retinal organoids and fetal retina. Different colors represent the expression value as log₂ transformed FPKM values. Blue to red represents low to high gene expression. FPKM, fragments per kilobase of exon per million fragments mapped.