Figure 2. AIM2 stabilizes Tregs to restrain autoimmunity.

a, Schematic of WT CD4⁺CD45RB^hi T cells (Tn)-induced colitis, with or without Tregs. b, Body weight of Rag1^–/– mice received Tn (n=8), Tn+WT Treg (n=8), Tn+Aim2^–/– Treg (n=9); two experiments, P value by two-way ANOVA and Holm -Sidak post-hoc test. c, Flow-cytometry of CD4⁺Foxp3⁺ Tregs in colons of Rag1^–/– mice that received Tn (n=6), Tn+WT Treg (n=6), Tn+Aim2^–/– Treg (n=9), 7 weeks after transfer, two experiments. P value by one-way ANOVA with Tukey’s multiple comparisons test. d, Schema for gene targeting to generate floxed Aim2 mice. e, Schema for generating Foxp3 Treg lineage tracing mice (upper). Tconv, stable Treg, and exTreg based on Foxp3 and Tomato expression (lower). f, EAE scores of mice of indicated genotypes (n=7/group). Two experiments, P value by two-way ANOVA and Holm-Sidak post-hoc test. g, Flow-cytometry of Foxp3 expression in CD4⁺ cells in PLN, spleen, and spinal cord (SC) of mice of indicated genotypes at day 28 of EAE, n=3 experiments. P value by multiple t test corrected by the Holm-Sadik method. h, Flow-cytometry of IFNγ and IL-17A production in CD4⁺Tomato⁻ T cells in spinal cords of mice of indicated genotypes at day 28 of EAE, n=3 experiments. P value by Multiple t test corrected by Holm-Sadik method. i, Flow-cytometry of Foxp3 production in CD4⁺Tomato⁺ T cells in spinal cords of mice of indicated genotypes at day 28 of EAE, n=3 experiments. P value by unpaired t test. j, Flow-cytometry of IFNγ and IL-17A production in CD4⁺Tomato⁺ T cells in spinal cords of mice of indicated genotypes at day 28 of EAE, n=3 experiments. P value by Multiple t test corrected by Holm-Sadik method. Data are means ± SEM, P values: ns, not significant, *P<0.05, **P<0.01, ****P<0.0001.