Table 5.
Hematological analysis of rat blood on day 5 and day 12 after administration of test samples
| |
Neutrophil (k/μL) |
Platelet (k/μL) |
||||
|---|---|---|---|---|---|---|
| 50 mpk | 100 mpk | 200 mpk | 50 mpk | 100 mpk | 200 mpk | |
| Day 5 | ||||||
| PBS | - | - | 3.83 (0.74) | - | - | 857.17 (42.85) |
| trastuzumab | - | - | 3.90(0.85) | - | - | 847.60 (114.75) |
| MMAF | - | - | 3.73 (0.99) | - | - | 904.83 (60.79) |
| T-N-F | 5.84 (1.54) | 4.42 (0.59) | 4.82 (1.74) | 623.67 (292.17) | 1027.83 (124.40) | 680.20 (113.27) |
| T-C-F | 4.01 (1.17) | 2.75 (0.48) | 1.28 (0.77) | 585.67 (284.16) | 495.80 (83.21) | 113.80(15.45) |
| T-K-F | 7.53 (3.91) | 6.64 (2.40) | 7.95 (1.79) | 586.00 (53.81) | 344.17 (83.08) | 208.17(44.75) |
| Day 12 | ||||||
| PBS | - | - | 1.91 (0.36) | - | - | 495 (141.22) |
| trastuzumab | - | - | 2.86 (1.13) | - | - | 477.50 (85.32) |
| MMAF | - | - | 1.94 (0.36) | - | - | 588.83 (91.03) |
| T-N-F | 3.31 (1.42) | 5.86 (1.63) | 5.92 (2.21) | 532.67 (92.86) | 566.83 (221.19) | 871.17 (152.08) |
| T-C-F | 4.72 (0.94) | 3.58 (0.87) | 27.13* | 484.50 (112.61) | 535.50 (167.18) | 335* |
| T-K-F | 5.09 (1.70) | 12.32** (4.14) | 18.96 (3.4) | 581.17 (206.21) | 537.80** (148.33) | 364.00 (113.70) |
* Data from only one survived animal
** One animal died after day 5
Numbers in parentheses denote standard deviation
mpk: mg per kg, MMAF: monomethyl auristatin F, T-N-F: trastuzumab-MMAF conjugate synthesized via NTERM conjugation, T-C-F: trastuzumab-MMAF conjugate synthesized via thiol conjugation, T-K-F: trastuzumab-MMAF conjugate synthesized via lysine conjugation, PBS: phosphate-buffered saline.