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. Author manuscript; available in PMC: 2021 Apr 28.
Published in final edited form as: Nat Immunol. 2020 Aug 31;21(10):1267–1279. doi: 10.1038/s41590-020-0765-7

Figure 4. NUR77/Nr4a1 selectively restrains humoral immune responses to T-independent II immunogens in vivo.

Figure 4.

A. Nr4a1+/+ and Nr4a1−/− mice were immunized IP with 100 μg NP-Ficoll and anti-NP IgG3 titers were determined via ELISA at serial time points. Data in A are representative of N=5 biological replicates, and 3 independent experiments. B. MB1-Cre and Nr4a1cKO mice were immunized with 100 μg NP-Ficoll and anti-NP IgM titers were determined via ELISA at serial timepoints. Data in B are representative of N=5 biological replicates. C,D Nr4a1+/+ and Nr4a1−/− mice were immunized IP with 100 μg NP-LPS and anti-NP IgG3 (C) and IgM (D) titers were determined via ELISA at serial time points. Data in D, E are representative of N=4 biological replicates. E,F. Nr4a1+/+ and Nr4a1−/− mice were immunized IP with 100 μg NP-KLH admixed 1:1 with alum and anti-NP IgG1 were determined via ELISA at serial time points. ELISA plates were coated with either NP-24-BSA (E), or NP-1-RSA (F) in order to detect total and high affinity NP-specific Abs respectively. Data in E, F are representative of N=4 biological replicates and 2 independent experiments. Mean +/− SEM displayed for all graphs. Statistical significance was assessed with two-tailed unpaired student’s t-test with Holm-Sidak (A-F). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001