Figure 3.

Enhanced production of Chlamydia-specific Th1, Th2, and Th17 cytokines by T-cells from PLGA-rMOMP-immunized and challenged mice. Immunomagnetic purified splenic T-cells (1×10⁶) were co-cultured with mitomycin-C treated APCs (1×10⁶) and stimulated with rMOMP (5 μg/mL) for 48 h at 37°C in a 5% CO₂ humidified atmosphere. Cell-free supernatants were collected by centrifugation and used for quantification of cytokines; IFN-γ (Th1) (A), IL-2 (Th1) (B), IL-10 (Th2) (C), and IL-17 (Th17) (D) using cytokine specific ELISAs. Immunized mice (pre); immunized-challenged and re-challenged mice (post). Each bar represents the mean ± standard deviation (SD) of triplicates from each sample. Statistical analyses were performed using two-way ANOVA followed by Tukey’s multiple comparisons and significant differences were considered at ****P < 0.0001, ***P < 0.001, **P < 0.01 and *P < 0.05.