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ATP binds wild-type PLC-γ1 as measured by isothermal titration calorimetry. Thermogram (top) and binding isotherm (bottom) for ATP titrated into wild-type PLC-γ1 in a buffer containing 50 mM HEPES (pH 7.4), 150 mM NaCl, 1 μM CaCl2, and 2 mM TCEP.
