Table 3. Top 20 peptides identified for each infection.
This table reports the 20 peptides with the highest Δ-values for each infection. The Δ-values were calculated by subtracting the average intensity (in relative light units (RLU)) in healthy controls (HC) from the average intensity (in RLU) in the infected group. The number of positive samples (i.e. signal > 5,000 RLU) for both the infected group (n = 10 for A. lumbricoides, n = 11 for T. trichiura, n = 9 for hookworm and n = 7 for S. mansoni) and the healthy control group (n = 24) is indicated for each of the peptides listed.
| Species | Peptide ID | Uniprot Protein name | Peptide sequence | Number of positive samples (>5,000 RLU) | RLU Range Infected | RLU Range Healthy Controls | Δ (Avg. Infected–Avg. HC) | |
|---|---|---|---|---|---|---|---|---|
| Infected | Healthy controls | |||||||
| A. lumbricoides | ALUE_0000851001_mRNA_1;551 | Unknown | AKQDSFISNESTKLH | 8 | 1 | 26–46837 | 101–5111 | 21908 |
| Smp_034080.1;353 | Putative erythrocyte membrane protein | GIEAIKQNDESEFPNN | 8 | 1 | 294–48867 | 31–8289 | 18297 | |
| NECAME_06710;144 | HEAT repeat protein | INESSMKQASLISNVM | 7 | 1 | 19–49175 | 23–5893 | 15963 | |
| ALUE_0001246601_mRNA_1;617 | DNA-directed RNA polymerase III subunit RPC3 | ERQLKMESIIANIEAD | 6 | 0 | 37–44595 | 41–3581 | 15640 | |
| ANCDUO_02559;123 | Zinc finger, C3HC4 type | GIDEAVLRRTVDDLLT | 9 | 0 | 2951–47823 | 30–4345 | 14554 | |
| GS_23998;441 | Unknown | LIERQLKMESIIANIE | 6 | 0 | 20–41166 | 22–3109 | 14321 | |
| Smp_152630.1;1 | MEG-12 | GENYEQQLQQPKAYGI | 4 | 0 | 10–62200 | 22–1453 | 13232 | |
| TTRE_0000850001_mRNA_1;188 | 205 kDa Pk1(B+)1+ SICAvar antigen | GVTANDLRTAEAMVRS | 6 | 0 | 8–46446 | 19–571 | 13109 | |
| NECAME_05279;46 | Uncharacterized protein | MAKEDGLISNLRSYPP | 7 | 1 | 49–41397 | 22–5999 | 13088 | |
| Smp_063110.2;144 | Putative zinc finger protein | GIEQQVILQPAAPVAM | 6 | 0 | 240–40053 | 22–3087 | 12608 | |
| NECAME_07428;155 | PAP2_C domain-containing protein | GIEQLRPRELCGDLIV | 7 | 0 | 962–34212 | 26–4284 | 12265 | |
| GS_23620;782 | Unknown | GIEEMTPSKKANNTAG | 8 | 1 | 68–45073 | 28–8035 | 12252 | |
| Smp_103170.1;78 | Unknown | PPNYTPERYLEMGNRN | 4 | 1 | 431–51442 | 188–8920 | 12195 | |
| Smp_151380.1;507 | Anaphase-promoting complex subunit 1 | KTESLISNSPSTKKSK | 6 | 0 | 9–40354 | 22–2015 | 12078 | |
| ALUE_0000793101_mRNA_1;694 | Unknown | GIEKIREKLNPFVVEK | 5 | 1 | 182–51442 | 50–10302 | 11861 | |
| NECAME_00764;3928 | Uncharacterized protein | LKKIIGGHLQMPTAIV | 5 | 0 | 6–57516 | 20–386 | 11605 | |
| ANCDUO_18503;111 | Uncharacterized protein | SKFDSVILNGYNIVGD | 6 | 0 | 5–50886 | 20–895 | 11425 | |
| TTRE_0000749301_mRNA_1;12 | Unknown | KTVPPRKKDSEISNSV | 6 | 0 | 91–32314 | 26–2676 | 11359 | |
| GS_00707;166 | Unknown | GIDRVIDAFRNAYTQV | 4 | 0 | 19–33437 | 23–1963 | 11106 | |
| NECAME_01953;144 | Uncharacterized protein | KNLISAQLNQTATGQP | 6 | 0 | 1131–39427 | 36–4993 | 10934 | |
| T. trichiura | ALUE_0000851001_mRNA_1;551 | Unknown | AKQDSFISNESTKLH | 10 | 1 | 4645–48005 | 101–5111 | 21749 |
| ANCDUO_02559;123 | Zinc finger, C3HC4 type | GIDEAVLRRTVDDLLT | 11 | 0 | 5567–47823 | 30–4345 | 21584 | |
| Smp_034080.1;353 | Putative erythrocyte membrane protein | GIEAIKQNDESEFPNN | 10 | 1 | 294–38372 | 31–8289 | 21424 | |
| NECAME_01953;144 | Uncharacterized protein | KNLISAQLNQTATGQP | 6 | 0 | 44–53181 | 18–2211 | 16910 | |
| GS_00707;166 | Unknown | GIDRVIDAFRNAYTQV | 8 | 0 | 1356–39427 | 36–4993 | 15994 | |
| NECAME_06710;144 | HEAT repeat protein | INESSMKQASLISNVM | 8 | 1 | 733–43011 | 23–5893 | 15854 | |
| Smp_152630.1;1 | MEG-12 | GENYEQQLQQPKAYGI | 5 | 0 | 8–61569 | 22–1453 | 15837 | |
| ALUE_0001246601_mRNA_1;617 | DNA-directed RNA polymerase III subunit RPC3 | ERQLKMESIIANIEAD | 7 | 0 | 646–45073 | 41–3581 | 14147 | |
| GS_23620;782 | Unknown | GIEEMTPSKKANNTAG | 8 | 1 | 68–49508 | 28–8035 | 13550 | |
| ANCDUO_21963;35 | Uncharacterized protein | GVEHLARVNCVLWQND | 7 | 0 | 485–46611 | 52–4998 | 13079 | |
| TTRE_0000257301_mRNA_1;122 | Unknown | GIDERLAHVWQKTTNA | 6 | 1 | 1231–45116 | 59–10335 | 12956 | |
| GS_23998;441 | Unknown | LIERQLKMESIIANIE | 7 | 0 | 440–40911 | 22–3109 | 12878 | |
| Smp_063110.2;144 | Putative zinc finger protein | GIEQQVILQPAAPVAM | 9 | 0 | 240–34765 | 22–3087 | 12661 | |
| ANCDUO_10263;496 | Putative polyribonucleotide nucleotidyltransferase | GIDHVLDKMAVMLDRP | 8 | 1 | 2039–40107 | 25–6745 | 12588 | |
| ANCDUO_05567;1 | Uncharacterized protein | MEIANGKEWKSDVRET | 5 | 0 | 20–51748 | 23–2684 | 12323 | |
| GS_00126;243 | Unknown | GVLEMIKKYLINYEHM | 5 | 1 | 7–50732 | 32–5119 | 12294 | |
| NECAME_02686;166 | Unknown | DKIVSEASRYKDMLQF | 5 | 1 | 366–54645 | 60–7441 | 12170 | |
| GS_08916;309 | Unknown | GIDDIDEDELECILAN | 7 | 1 | 3027–40053 | 33–8529 | 12008 | |
| TTRE_0000898701_mRNA_1;67 | Unknown | GTVYKLQEMKPSVVKE | 4 | 0 | 4–51748 | 20–895 | 11972 | |
| ALUE_0001542901_mRNA_1;23 | Unknown | ITCKVTGRYTSALENL | 6 | 0 | 26–60526 | 19–941 | 11783 | |
| Hookworm | Smp_034080.1;353 | Putative erythrocyte membrane protein | GIEAIKQNDESEFPNN | 8 | 1 | 3894–36306 | 31–8289 | 18206 |
| ANCDUO_02559;123 | Zinc finger, C3HC4 type | GIDEAVLRRTVDDLLT | 8 | 0 | 1097–37428 | 30–4345 | 17623 | |
| ALUE_0000851001_mRNA_1;551 | Unknown | AKQDSFISNESTKLH | 5 | 1 | 26–44812 | 101–5111 | 13798 | |
| GS_23620;782 | Unknown | GIEEMTPSKKANNTAG | 7 | 1 | 1542–34806 | 28–8035 | 11960 | |
| ALUE_0002154501_mRNA_1;221 | Uncharacterized protein | FKELANKLLNKQQLAE | 6 | 1 | 932–52374 | 44–5966 | 11891 | |
| ALUE_0001868101_mRNA_1;34 | Unknown | SLAKKLFGSSAGLNDD | 4 | 0 | 183–43591 | 30–1890 | 11671 | |
| ANCDUO_21963;35 | Uncharacterized protein | GVEHLARVNCVLWQND | 7 | 0 | 485–30867 | 52–4998 | 11594 | |
| GS_15548;34 | Unknown | SLAKKLFGSSAGLNDD | 4 | 0 | 183–43591 | 32–2488 | 11582 | |
| GS_00707;166 | Unknown | GIDRVIDAFRNAYTQV | 5 | 0 | 1942–31701 | 36–4993 | 11247 | |
| ALUE_0000733401_mRNA_1;298 | Unknown | AKNGRQLYDNYIEARK | 4 | 0 | 126–32809 | 27–3427 | 11236 | |
| GS_08916;309 | Unknown | GIDDIDEDELECILAN | 7 | 1 | 1710–40053 | 33–8529 | 11231 | |
| GS_01502;573 | Unknown | KNGRQLYDNYIEARKR | 4 | 0 | 78–40862 | 28–1451 | 10892 | |
| ANCDUO_10263;496 | Putative polyribonucleotide nucleotidyltransferase | GIDHVLDKMAVMLDRP | 6 | 1 | 1227–40107 | 25–6745 | 10755 | |
| ANCDUO_06401;122 | Uncharacterized protein | GPWHQKLTAKKVAKSM | 4 | 1 | 15–38687 | 20–5099 | 10624 | |
| ANCDUO_05567;1 | Uncharacterized protein | MEIANGKEWKSDVRET | 3 | 0 | 9–51748 | 23–2684 | 10587 | |
| ALUE_0001434301_mRNA_1;111 | Unknown | QDILNNKALLGDEKAR | 4 | 0 | 13–57516 | 19–941 | 10349 | |
| TTRE_0000259701_mRNA_1;67 | Unknown | ARAGLQGDLLGLNVLC | 4 | 0 | 6–38452 | 19–2095 | 10332 | |
| NECAME_02954;232 | Beta-lactamase | VATKKLIGDQMLNRLQ | 4 | 0 | 159–33821 | 23–863 | 10284 | |
| TTRE_0000798101_mRNA_1;166 | Unknown | ILKKHDKVLQNTAGNE | 3 | 0 | 43–43168 | 19–2709 | 10235 | |
| ANCDUO_24637;1 | COesterase domain-containing protein | MTKPVKKTRWHQELSA | 4 | 0 | 7–39427 | 21–3782 | 10169 | |
| S. mansoni | Smp_152630.1;1 | MEG-12 | GENYEQQLQQPKAYGI | 6 | 0 | 4443–62200 | 22–1453 | 33784 |
| NECAME_01953;144 | Uncharacterized protein | KNLISAQLNQTATGQP | 5 | 0 | 262–53181 | 18–2211 | 32222 | |
| TTRE_0000898701_mRNA_1;67 | Unknown | GTVYKLQEMKPSVVKE | 6 | 0 | 2496–51748 | 20–895 | 27482 | |
| ALUE_0000851001_mRNA_1;551 | Unknown | AKQDSFISNESTKLH | 6 | 1 | 676–48005 | 101–5111 | 25208 | |
| ANCDUO_19104;23 | Sas10 domain-containing protein | GTVRKEMQKYSGESRG | 6 | 0 | 1313–44286 | 27–2391 | 21927 | |
| NECAME_06710;144 | HEAT repeat protein | INESSMKQASLISNVM | 5 | 1 | 180–43011 | 23–5893 | 20615 | |
| NECAME_10074;1464 | Uncharacterized protein | IELDPSAATSNTLHDV | 4 | 0 | 85–62200 | 18–2359 | 20462 | |
| Smp_162000.1;6582 | UBR-type domain-containing protein | QKIAISEELNAKQINS | 5 | 0 | 383–51442 | 28–1930 | 20002 | |
| NECAME_00764;3928 | Uncharacterized protein | LKKIIGGHLQMPTAIV | 5 | 0 | 9–57516 | 20–386 | 19697 | |
| ALUE_0001246601_mRNA_1;617 | DNA-directed RNA polymerase III subunit RPC3 | ERQLKMESIIANIEAD | 4 | 0 | 146–45073 | 41–3581 | 19667 | |
| TTRE_0000164301_mRNA_1;56 | Unknown | FVVIENLSNDEKQIFT | 5 | 0 | 176–41166 | 28–2467 | 19404 | |
| TTRE_0000806301_mRNA_1;342 | Unknown | ELELDPAKTNVNGGAI | 5 | 0 | 179–60104 | 18–3029 | 19162 | |
| ALUE_0001256701_mRNA_1;1255 | Unknown | KKLISEALNSETRLQN | 5 | 0 | 466–39540 | 27–3464 | 18956 | |
| Smp_034080.1;353 | Putative erythrocyte membrane protein | GIEAIKQNDESEFPNN | 4 | 1 | 2764–39968 | 31–8289 | 17679 | |
| ALUE_0000089701_mRNA_1;89 | Uncharacterized protein | GTVLKFPSLSPFANAA | 5 | 1 | 66–40220 | 26–6676 | 17505 | |
| GS_00094;969 | Unknown | FVVKEKRNANQFDVNN | 4 | 1 | 445–42092 | 136–13481 | 17494 | |
| TTRE_0000788501_mRNA_1;287 | Unknown | YKHVIGIDLNKEAIEN | 4 | 0 | 306–39209 | 26–2762 | 16791 | |
| TTRE_0000483901_mRNA_1;89 | Unknown | VSKQTISAHLKPLGSN | 4 | 1 | 28–59047 | 22–6293 | 16727 | |
| TTRE_0000134801_mRNA_1;243 | Unknown | SPLIGKEIVSLSLNGD | 4 | 0 | 31–46525 | 22–687 | 16646 | |
| ANCDUO_07179;45 | REX1 DNA Repair | IDREIVRILEEKLDHL | 5 | 0 | 606–42756 | 27–1848 | 16589 | |