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. Author manuscript; available in PMC: 2021 Apr 28.
Published in final edited form as: Nat Cancer. 2021 Jan 13;2(1):98–113. doi: 10.1038/s43018-020-00161-w

Extended Data Fig. 3: Expression and functional analysis of IL17 receptor and CARD 11 dependent signaling in T cells.

Extended Data Fig. 3:

a, RNA expression level of IL17RC, essential for a functional IL17 receptor68, in T cells and monocytes represented from DICE (Database of Immune Cell Expression, Expression quantitative trait loci and Epigenomics). b, NF-κB-GFP reporter activity in transduced Jurkat cells after stimulation with 200 ng/mL IL17A. Results are reported as mean of technical replicate values (bar) from 1 independent experiment with individual values (white circles), n=1. c, Immunoblot analysis of JPM50.6 CARD11 knockout and JPM50.6 cells reconstituted by CARD11-HA expression. Expression levels were verified for each independent experiment. d, NF-κB-GFP reporter activity after stimulation with 25 nM of PMA or 20 ng/mL TNFα as in c. e, Western blot analysis of FYN-TRAF3IP2-V5 and BCL10 expression in Jurkat cells and Jurkat BCL10 knockout cells infected with empty vector or FYN-TRAF3IP2-V5 expressing lentiviruses; and RT-PCR analysis of FYN-TRAF3IP2-V5 mRNA in the same Jurkat BCL10 knockout cells infected with empty vector or FYN-TRAF3IP2-V5 expressing lentiviruses. * and ** indicate non-specific bands detected by the V5 antibody, while the arrowhead indicates the location of the specific FYN-TRAF3IP2-V5 band detected with the same antibody. Expression levels were verified for each independent experiment.