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. Author manuscript; available in PMC: 2021 Apr 28.
Published in final edited form as: Nat Cancer. 2021 Jan 13;2(1):98–113. doi: 10.1038/s43018-020-00161-w

Figure 2. FYN-TRAF3IP2 drives NF-κB activation in response to TCR-induced PKC signaling.

Figure 2.

a, Immunoblot analysis of Jurkat NF-κB-GFP reporter cells lentivirally transduced with empty vector or with viruses driving expression of wild type TRAF3IP2-V5 or the FYN-TRAF3IP2-V5 fusion. Expression levels were verified in 3 independent experiments. b, NF-κB-GFP reporter activity in Jurkat cells as in a after stimulation with anti-CD3 (a-CD3) antibody. Results are reported as mean of fold change over empty vector IgG control from n=3 independent experiments ± s.d. Individual values are shown as white circles. P values were calculated using two-way ANOVA and Tukey’s or Sidak’s multiple comparison test. c, NF-κB-GFP reporter activity in Jurkat cells as in a after treatment with increasing doses of PMA. Bar graphs indicate the mean of technical replicate values obtained in parallel. Individual values are shown as white circles. The experiment was performed once.