Figure 5. Expression of FYN-TRAF3IP2 in mouse hematopoietic progenitors induces PTCL NOS.

a, Kaplan-Meier survival curves of mice transplanted with bone marrow progenitor cells from CD4 Cre-ERT2 Tet2^fl/fl mice retrovirally transduced to express GFP only, wild type TRAF3IP2 and GFP, or fusion FYN-TRAF3IP2 and GFP treated with vehicle (blue arrow) 8 weeks post-transplant. 10 mice were transplanted per experimental group (n=number of mice). Black arrows indicate the red blood cell immunizations. b, Splenic weight of animals at the endpoint after transplantation as in a. Red circles, GFP⁺ CD4⁺ lymphoma; black circles, no lymphoma; gray circle, GFP⁺ CD8⁺ lymphoma; horizontal line, mean value. P values were calculated using two-tailed‎ Student’s‎ t-test. c, Kaplan-Meier survival curves of mice transplanted with bone marrow progenitor cells from CD4 Cre-ERT2 Tet2^fl/fl mice retrovirally transduced to express GFP only, wild type TRAF3IP2 and GFP, or fusion FYN-TRAF3IP2 and GFP treated with tamoxifen (red arrow) 8 weeks post-transplant. 10 mice were transplanted per experimental group (n=number of mice). Black arrows indicate the red blood cell immunizations. d, Splenic weight of animals at the endpoint after transplantation as in c. Red circles, GFP⁺ CD4⁺ lymphoma; black circles, no lymphoma; horizontal line, mean value. P values were calculated using two-tailed‎ Student’s‎ t-test. e, Spleens from FYN-TRAF3IP2-induced lymphoma-bearing mice (bottom) and nine representative mice that were lymphoma-free at the end of follow up (top) and representative lymph nodes from FYN-TRAF3IP2-induced lymphoma-bearing mice. f, Representative FACS plots showing GFP⁺ CD4⁺ FYN-TRAF3IP2 lymphoma cells infiltrating lymph nodes and bone marrow. g, Histological micrographs of hematoxylin-eosin stained spleen sections of immunized control mice and FYN-TRAF3IP2-induced CD4⁺ PTCL, NOS. Tissues from 3 mice per group showed similar results. Scale bar = 400 μm.