Fig. 5.

DNA adducts in the Phenion® FT skin model. Tissues were treated three times (48, 24 and 3 h) with 2-AAF or BaP ± APC or 1 time (3 h) with 2-AAF, N-OH-2-AAF or N-OH-2-AAF (see figure legends for treatment times). The left panels (a–e) are representative autoradiographic profiles of the DNA adducts formed in Phenion®FT skin tissues after exposure. The origins, at the bottom left-hand corners on the TLC plates, were cut off before imaging and the arrows indicate the adduct spots used in the quantitation of the adduct levels shown in the graphs in the right panels (f–h). All values (n = 3) are mean ± SD. DNA adduct levels are calculated per 10⁸ normal nucleotides. *P < 0.05, **P < 0.01. P values for specific group comparisons are shown within brackets. No statistical comparisons to the SC group were done as 2-AAF-related DNA adducts in the controls were not detectable (ND). PC: BaP. Note: the adduct spots found from exposure to 2-AAF, N-OH-2-AAF or N-OH-2-AF are not distinguishable on TLC.