Figure 13.

Effects of total flavonoids of Rhizoma Drynariae (TFRD) on NF-κB p65 and IκB-α levels in IL-1β-induced SW1353 cells. Before stimulation with 10 ng/mL IL-1β for 30 min, cells were pretreated with 150, 300, or 600 μg/mL TFRD for 1 h. Total cytosolic and nuclear proteins were extracted. IκB-α in the cytosol and NF-κB p65 in the nucleus were measured by Western blotting. Lamin B and β-actin were used as internal references for the nuclear and cytoplasmic fractions, respectively. The data are derived from three independent experiments and expressed as the mean ± standard deviation. (^∗p < 0.05 compared to the control group; ^#p < 0.05 compared to the IL-1β-treated group).