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Isolation, culture, and identification of primary SMNs. (a) Spinal cords were dissected from the 14-day-old mouse embryos. Scale bars = 50 mm. Representative images of SMNs on (b) Day 3, (c) Day 5, and (d) Day 7. Primary SMNs were labeled with (e) Tuj1, (f) MAP-2, (g) ChAT, and (h) GFAP. Scale bars = 50 μm.
