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. 2021 Apr 20;2021:5524344. doi: 10.1155/2021/5524344

Figure 2.

Figure 2

circ-ITCH inhibits ccRCC cell metastasis via sponging miR-106b-5p. (a) The predicted binding sites between circ-ITCH and miR-106b-5p. (b) The dual luciferase reporter assay detects the luciferase activity of circ-ITCH-WT/circ-ITCH-MUT in OSRC-2 and SW839 cells cotransfected with miR-106b-5p mimics. (c) The expression of miR-106b-5p in ccRCC tissues. (d, e) Transwell assay detects migration and invasion abilities of OSRC-2 and SW839 cells transfected with OE-NC, OE-circ-ITCH, OE-circ-ITCH, and miR-106b-5p mimic. (f) Wound healing detects migration abilities of OSRC-2 and SW839 cells transfected with sh-NC, sh-circ-ITCH, and sh-circ-ITCH + miR-106b-5p inhibitor. (g) Western blot analyzes the expressions of E-cadherin, N-cadherin in OSRC-2, SW839 cells transfected with NC, OE-circ-ITCH, OE-circ-ITCH, and miR-106b-5p mimic.