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. 2021 Apr 7;592(7856):768–772. doi: 10.1038/s41586-021-03425-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — Cell-suspension-culture protoplasts were transfected with pJIT60-2xp35SS:NLS:AuxSen and the FRET response was captured by flow cytometry as the ratio of the emission between the Aquamarine peak intensity (FL9) and mNeonGreen peak intensity (FL10) as ‘Log_Height’ (a.u.). a, Normalized excitation and emission spectra for mNeonGreen and Aquamarine. The 405-nm laser line is shown as a vertical bar and bandpass filters are shown as open boxes, as rendered by FPBase⁴⁴. As FRET occurs, the emission output is shifted from the FL9 to the FL10 bandpass. b, Baseline level of AuxSen FRET response, without exogenous IAA. c, Maximal response level of AuxSen with 10 mM IAA.