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. 2021 Apr 7;592(7856):768–772. doi: 10.1038/s41586-021-03425-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b, Change in the nuclear FRET ratio (colour bar) within the root tip of individual seedlings treated with brefeldin A (BFA) or DMSO. a, Images. b, Quantitative analysis after treatment with BFA (10 μM for 10 h, magenta; n = 10) or DMSO (control, green; n = 9). The thick lines represent the mean (±s.e.m.), the thin lines each represent individual seedlings. c, d, The response of AuxSen to root gravitropism. c, Top, nuclear FRET ratio (colour bar) before and after turning (first and second images), and before and after turning back to the near-vertical position (third and fourth images). Bottom, Cartoons of the seedlings, with arrows indicating the direction of the gravity vector. The signal moves from the left side to the bottom and back to the left side; the colour scale indicates the relative FRET ratio. d, Quantified response of the sensor in individual roots. The thick lines represent the mean (±s.e.m.), the thin lines each represent individual roots (n = 10). Scale bars, 100 μm (a, c). e, Diagram of root tip. Nuclei within 100 μm above the quiescent centre (asterisk) and at least 10 μm from the midline (red line) were analysed (dark triangles). Green, right/bottom; magenta, left/top.