Extended Data Table 2.

Dissociation constants and overview of crystallographic data for TrpR and selected variants

a, Dissociation constants of selected variants for TRP, IAA, IAN and PAA as determined by ITC. Errors are calculated from fits of two technical replicates. n.d. indicates that the data was measured but the fit did not converge owing to low affinity, whereas for later variants TRP measurements were omitted altogether (n.a.). The dissociation constants were determined via ITC, at 20 °C and pH 8.0. Thermodynamic values for enthalpy (ΔH), entropy (−TΔS), and free energy (ΔG) are shown in kcal mol⁻¹. Stoichiometry is given as N. Data are mean ± s.d., derived from 2 technical replicates.

b, Overview of crystallographic data for TrpR and its variants in complex with ligands as indicated.