Extended Data Fig. 2. Gel-filtration analysis of the rNLRP1–rDPP9 and the CARD8–hDPP9 interactions.

a, Full-length GST–rNLRP1 and rDPP9 were expressed in insect cells. The complex was purified through GS4B resin. After elution, GST was removed using precision protease and the complex was subjected to gel filtration. Left, the gel-filtration profile of the complex. Right, the protein fractions were visualized by SDS–PAGE followed by Coomassie-blue staining. b, The interaction between full-length GST–CARD8 and hDPP9 was assayed as described in a. See Supplementary Fig. 1 for gel raw data.