Skip to main content
. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: J Allergy Clin Immunol. 2020 Oct 29;147(5):1936–1948.e9. doi: 10.1016/j.jaci.2020.10.024

FIG 2. Increased ceramide staining in HDM-challenged mice co-localizes with cleaved caspase 3 in lung epithelium.

FIG 2.

(A) Mice were challenged i.n. with HDM or saline and lungs as indicated and examined on day 15 as described in Fig 1. Lung sections were stained with anti-ceramide antibody (red), anti-cleaved caspase 3 antibody (green). Lower panels are zoom boxes indicated in middle panels. (B) Lung sections were stained with anti-ceramide antibody (red), anti-cleaved caspase 3 antibody (green) and anti-cytokeratin 18 (yellow), a marker for epithelial cells. (A,B) All sections were co-stained with DAPI to visualize nuclei (blue). Co-localization is shown in the overlay panels. Size bar: 50 or 10 μm as indicated.