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. 2021 Apr 15;8:644224. doi: 10.3389/fvets.2021.644224

Figure 5.

Figure 5

IgG-cleaving activity of r0376. (A) SDS-PAGE assay detects the effect of r0376 cleaving bovine IgG. M, Molecular mass marker; Lane 1, bovine IgG incubated with r0375; Lane 2, bovine IgG incubated with r0375 and r0376; Lane 3, bovine IgG incubated with r0376. (B) Western blotting detects the full-length or cleaved heavy chain of bovine IgG. Lane 1, bovine IgG incubated with r0375; Lane 2, bovine IgG incubated with r0375 and r0376; Lane 3, bovine IgG incubated with r0376. Black star, cleaved IgG heavy chain fragment.