Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 15;12:607641. doi: 10.3389/fimmu.2021.607641

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Qiu, Luo, Ma, Saminathan, Li, Gunnersen, Gelbard and Hammond

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Truncated Sez6L2 does not act as a cofactor for Factor I cleavage of C4b. (A) Schematic of Factor I plus cofactor cleavage of C4b. (B) C4b and FI were incubated alone, with concentrations of Sez6L2-MH ranging from 1 to 8 µM, or with 1 µM C4BP for two hours at 37°C. Then samples were then run and visualized on Coomassie stained gels. Incubation of C4b and FI with Sez6L2-MH did not result in the appearance of C4b cleavage products C4d, C4α3, or C4α4. On the other hand, C4BP (a known cofactor of FI for C4b cleavage) supported FI’s production of C4d, C4α3, and C4α4. (C) Sez6L2-MH does not support Factor I cleavage of C4b even with increased time. C4b and FI were incubated with 1.5 µM Sez6L2-MH for 0.25-8 hours and then visualized on Coomassie stained gels.