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. 2021 Apr 18;23(5):461–472. doi: 10.1016/j.neo.2021.03.009

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 5 — Inhibiting CTH decreases xCT stability in vivo. (A) Immunoblots showing decreased CTH expression in HCT-116 shCTH cells. (B) Mice injected with shCTH cell lines exhibited decreased tumor volume compared with control animals injected with HCT-116 cell lines transfected with negative control plasmids. (C) IHC images showing decreased xCT and Ki67 intensity in shCTH tumors. (D) Decreased H₂S levels in shCTH tumors. (E) Modified biotin switch assays showing decreased persulfidation of OTUB1 in shCTH tumors. (F) Immunoblots following IP showing decreased xCT levels and decreased conjunction between OTUB1 and xCT in shCTH tumors. (G) Decreased GSH levels in shCTH tumors. (H) Quantitative real-time PCR showing increased PTGS2 mRNA levels in shCTH tumors. (*P < 0.05 vs Control).