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. 2021 Apr 15;11:646559. doi: 10.3389/fonc.2021.646559

Figure 2.

Figure 2

LINC00518 affects LUAD cell proliferation by regulating the cell cycle. (A) A549 and PC9 cells were transfected with si-LINC00518 respectively, while SPCA1 cells were transfected with pcDNA-LINC00518. The results were validated by qRT-PCR.NC and blank were used as controls. (B) CCK-8 assay was applied to detect the proliferation of A549,PC9 and SPCA1 cells transfected with si-LINC00518 or NC. (C, D) Colony formation analysis and Edu assays in A549, PC9 and SPCA1 cells transfected with si-LINC00518,pcDNA-LINC00518 or NC. (E) Cell cycle distributions were analyzed in A549, PC9 and SPCA1 cells, respectively by Flow Cytometry. All data are expressed as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.