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. 2021 Apr 15;11:646559. doi: 10.3389/fonc.2021.646559

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Copyright © 2021 Han, Wu, Zhang, Song, Shi and Chang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MECP2 is a target of miR-185-3p regulated by LINC-00518 in LUAD cells. (A) The binding sites of miR-185-3p on the 3′-UTR of MECP2. (B) Relative expression of MRCP2 in LUAD tissues compared with adjacent normal tissues from the TCGA database. (C) The expression of MECP2 were detected by IHC from the HPA database. (D) Luciferase assay of LUAD cells transfected with MECP2-WT or MECP2-MUT reporter together with miR-185-3p or NC. (E, F) Relative mRNA and protein levels of MECP2 in A549, PC9 and SPCA1 cells transfected with NC, si-LINC00518 or pcDNA-LINC00518. Data are represented as mean ± SD. (*indicates P < 0.05, **P < 0.01).