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. 2021 Mar 4;296:100508. doi: 10.1016/j.jbc.2021.100508

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Immunofluorescence staining of 5xFAD and wild-type mouse brain sections using Aβ antibodies.A–D, mouse brain sections from the (A and B) frontal cortex and (C and D) hippocampus (CA1) were stained using conformational antibodies [clone 97 in (A) and (C) and aducanumab in (B) and (D)] at 200 nM, a sequence-specific Aβ antibody (NAB 228; recognizes Aβ1-11) at 1:200x dilution, and DAPI. The 5xFAD mice were 8 months old. Slides were imaged using Leica SP5 confocal microscope. The scale bars are 50 μm for the main images and 15 μm for the inset images.