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. 2021 Mar;42(3):230–237. [Article in Chinese] doi: 10.3760/cma.j.issn.0253-2727.2021.03.009

表1. 实时定量RT-PCR检测KEL、LRGL、circ-KEL表达引物序列.

基因 正向引物序列(5′→3′) 反向引物序列(5′→3′)
GAPDH CTGGGCTACACTGAGCACC AAGTGGTCGTTGAGGGCAATG
KEL GGATCTCCGGGATCATTACCT ATTCTCCGAAGTCGGTTTTTGT
LRG1 GTCCTCTTGGAGCAGACAG GGAACAGAGTTCTAGAAACATGG
U6 CTCGCTTCGGCAGCACA AACGCTTCACGAATTTGCGT
circ-KEL AACACCTCCCTGGTCACATC ATTTCATGGCTTAAGAGACAAGC