TABLE 6.

Autophagy protein genetic interventions and fasting.

References	Animal	Fasting period (hours)	Genetic intervention	Autophagic target	Autophagy modulation (up/down)	Steatosis outcome (up/down)	Correlation (+/0/−)
Singh et al., 2009	C57BL/6	24	Atg7 f/f; Alb-Cre mice (Atg7-Li KO)	ATG7	Down	Up	−
Shibata et al., 2009	not described	12, 24	Atg7 f/f; Alb-Cre mice (Atg7-Li KO)	ATG7	Down	Down	+
Kim et al., 2013	C57BL/6J	24	Atg7 f/f; Alb-Cre mice (Atg7-Li KO)	ATG7	Down	Down	+
Kwanten et al., 2014	C57BL/6J	24	Atg7 f/f; Alb-Cre mice (Atg7-Li KO)	ATG7	Down	Down	+
Takahashi et al., 2020	C57BL/6	24	Atg7 f/f; Alb-Cre mice (Atg7-Li KO)	ATG7	Down	Down	+
Saito et al., 2019	C57BL/6	not described	Atg7 f/f; Alb-Cre mice (Atg7-Li KO)	ATG7	Down	Down	+
Takagi et al., 2016	C57BL/6J	36	Atg5 f/f; Alb-Cre mice (Atg5-Li KO)	ATG5	Down	Down	+
Li et al., 2018	C57BL/6J	16	Atg5 f/f; Alb-Cre mice (Atg5-Li KO)	ATG5	Down	Down	+
Takahashi et al., 2020	C57BL/6	6-48	Atg5 f/f;Mx1-Cre (Atg5-Li conditional KO)	ATG5	Down	Down	+
Settembre et al., 2013	C57BL/6	24	TcfebloxP/loxP; Alb-Cre mice (Tfeb-Li KO)	TFEB	Down	Up	−
Ma et al., 2013	C57BL/6J	16	Fip200 f/f; Alb-Cre mice (Fip200-Li KO)	FIP200	Down	Down	+

Articles using genetic interventions in the autophagy machinery and fasting models to promote steatosis, describing fasting duration, liver steatosis, and LC3-II and p62 protein in levels in the liver in comparison to control groups (fed), normalized by a housekeeping protein. LC3-II and p62 levels were determined through SDS-PAGE Western blots in the manuscripts and described semi-quantitatively or qualitatively. All animals were male mice. Positive correlation = steatosis is up, and autophagy is up OR steatosis is down, and autophagy is down; Negative correlation = steatosis is up, and autophagy is down OR steatosis is down, and autophagy is up.